Regulatory

Part:BBa_K2967011:Design

Designed by: Rui Wang   Group: iGEM19_NEU_CHINA   (2019-09-28)


The inducible promoter, PhrpL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimize the sequence to make it work well in E coli.

Source

It comes from the hrp gene regulatory network for Type III secretion in Pseudomonas syringae.

References

Jovanovic,M., James,E.H., Burrows,P.C., Rego,F.G.M., Buck,M. and Schumacher,J. (2011) Regulation of the co-evolved HrpR and HrpS

AAA+ proteins required for Pseudomonas syringae pathogenicity.Nat. Commun., 2:177.